ENGINEERING STRATEGIES FOR ENHANCED RIBOFLAVIN PRODUCTION BY CANDIDA FAMATA YEAST

Abstract

Riboflavin is an essential compound for humans and animals. Industrial riboflavin production is achieved through the use of recombinant strains of the bacterium Bacillus subtilis and filamentous fungus Ashbya gossypii. Some mutants of Candida famata yeast also belong to the most famous riboflavin overproducers but there great flavinogenic potential remains unrealized. Metabolic engineering approaches have been successfully applied for construction of riboflavin overproducer in C. famata. A new factor of negative regulation of riboflavin biosynthesis in yeast C. famata was identified, which is encoded by the gene SEF2.. We are also focused on extension of the substrate range to produce the vitamin B2 such as whey and lignocellulose. Further studies should be accelerated for the effective utilization of substrates and supplements, facilitating better methods for bioconversion with an economic and industrial perspective.