OPTIMIZATION OF THE ISOLATION METHOD FOR EXOPOLYSACCHARIDES OF PROBIOTIC BACILLUS VELEZENSIS STRAINS

Abstract

This study focused on the implementation and adaptation of new approaches for extracting exopolysaccharides (EPS) from the probiotic bacterial strains Bacillus velezensis UCM B-7242 and B. velezensis UCM B-7243. The primary objective of this research was to optimize the EPS isolation method by eliminating the conventional alcohol precipitation stage to achieve the highest possible yield and purity of the exopolysaccharides. In the course of the study, five sequential extraction stages were conducted and optimized, resulting in concentrated EPS samples with a significantly reduced content of co-extracted impurities.