NOVEL POSITIVE SELECTION APPROACH FOR THE YEAST OGATAEA POLYMORPHA

Abstract

A novel positive selection approach for Ogataea polymorpha based on CAT8 overexpression and metformin-mediated growth inhibition was investigated. Overexpression of CAT8 conferred a growth advantage under selective conditions with glycerol as the carbon source, demonstrating the potential of this system for selection of recombinant O. polymorpha strains.